Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration

ChangDong Lin; ZhaoYuan Liu; Yue Li; JianFeng Chen

doi:10.1016/j.xpro.2021.100720

Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration

STAR Protoc. 2021 Aug 5;2(3):100720. doi: 10.1016/j.xpro.2021.100720. eCollection 2021 Sep 17.

Authors

ChangDong Lin¹, ZhaoYuan Liu¹, Yue Li¹, JianFeng Chen^{1

2}

Affiliations

¹ State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
² School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.

Abstract

Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to Lin et al. (2019).

Keywords: Cell Biology; Cell isolation; Immunology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Adhesion
Disease Models, Animal
Fever / diagnosis*
Hyperthermia / physiopathology
Hyperthermia, Induced / methods*
Inflammation
Mice
T-Lymphocytes / metabolism